Efficient mitosis detection: leveraging pre-trained faster R-CNN and cell-level classification.

The assessment of mitotic activity is an integral part of the comprehensive evaluation of breast cancer pathology. Understanding the level of tumor dissemination is essential for assessing the severity of the malignancy and guiding appropriate treatment strategies. A pathologist must manually perform the intricate and time-consuming task of counting mitoses by examining biopsy slices stained with Hematoxylin and Eosin (H&E) under a microscope. Mitotic cells can be challenging to distinguish in H&E-stained sections due to limited available datasets and similarities among mitotic and non-mitotic cells. Computer-assisted mitosis detection approaches have simplified the whole procedure by selecting, detecting, and labeling mitotic cells. Traditional detection strategies rely on image processing techniques that apply custom criteria to distinguish between different aspects of an image. Additionally, the automatic feature extraction from histopathology images that exhibit mitosis using neural networks.Additionally, the possibility of automatically extracting features from histopathological images using deep neural networks was investigated. This study examines mitosis detection as an object detection problem using multiple neural networks. From a medical standpoint, mitosis at the tissue level was also investigated utilising pre-trained Faster R-CNN and raw image data. Experiments were done on the MITOS-ATYPIA- 14 dataset and TUPAC16 dataset, and the results were compared to those of other methods described in the literature.

A Multi-Classifier System for Automatic Mitosis Detection in Breast Histopathology Images Using Deep Belief Networks.

Mitotic count is an important diagnostic factor in breast cancer grading and prognosis. Detection of mitosis in breast histopathology images is very challenging mainly due to diffused intensities along object boundary and shape variation in different stages of mitosis. This paper demonstrates an accurate technique for detecting the mitotic cells in Hematoxyline and Eosin stained images by step by step refinement of segmentation and classification stages. Krill Herd Algorithm-based localized active contour model precisely segments cell nuclei from background stroma. A deep belief network based multi-classifier system classifies the labeled cells into mitotic and nonmitotic groups. The proposed method has been evaluated on MITOS data set provided for MITOS-ATYPIA contest 2014 and also on clinical images obtained from Regional Cancer Centre (RCC), Thiruvananthapuram, which is a pioneer institute specifically for cancer diagnosis and research in India. The algorithm provides improved performance compared with other state-of-the-art techniques with average F-score of 84.29% for the MITOS data set and 75% for the clinical data set from RCC.

Detection of mitotic nuclei in breast histopathology images using localized ACM and Random Kitchen Sink based classifier.

The exact measure of mitotic nuclei is a crucial parameter in breast cancer grading and prognosis. This can be achieved by improving the mitotic detection accuracy by careful design of segmentation and classification techniques. In this paper, segmentation of nuclei from breast histopathology images are carried out by Localized Active Contour Model (LACM) utilizing bio-inspired optimization techniques in the detection stage, in order to handle diffused intensities present along object boundaries. Further, the application of a new optimal machine learning algorithm capable of classifying strong non-linear data such as Random Kitchen Sink (RKS), shows improved classification performance. The proposed method has been tested on Mitosis detection in breast cancer histological images (MITOS) dataset provided for MITOS-ATYPIA CONTEST 2014. The proposed framework achieved 95% recall, 98% precision and 96% F-score.